報告題目💁🏻‍♀️🤳🏽：IncHI2-Mediated Multiple Antimicrobial Resistance Dissemination in Salmonella enterica

報告人✡︎：施春雷

時  間： 2018年06月15日 星期五上午10:00-11:00

地  點： 閔行校區生物藥學樓樹華多功能廳（800號）

主持人𓀘：  楊廣宇 研究員

報告人簡介：

施春雷，EON体育4農業與生物EON4研究員。2006年在EON体育4獲生物醫學工程（生物技術）博士學位，在美國康奈爾大學食品科學系從事博士後研究。致力於食品微生物性安全基礎理論與控製技術的研究工作👹，重點開展了食源性致病菌菌膜形成分子機製基礎研究🧑🏻‍✈️，開發食源性致病菌快速檢測和精準分型技術，並應用於微生物風險評估，首次提出了菌膜形成的抗氧脅迫協同調控模型👨🏿。發表SCI論文47篇🤘，主持國家重點研發計劃項目一項👨🏽‍🚒🪒。

報告摘要✍🏼：

The widely usage of antimicrobials contributes to the increase in prevalence of antimicrobial-resistant Salmonella. And plasmid plays a critical role in horizontal transfer of antimicrobial-resistance markers in Salmonella. Here, we aimed to characterize plasmid profile responsible for antimicrobial-resistance markers and clarify genetic characteristics of antimicrobial-resistance plasmids. There are 44 Salmonella isolates were examined resistant to at least one antimicrobial with highest resistance against Ampicillin. These antimicrobial-resistant isolates were screened for plasmid-mediated quinolone resistance genes and β-lactamase resistance genes. The predominant plasmid replicon typing was IncHI2. After plasmid curing assay. The loss of IncHI2 plasmids was correlated with the loss of two groups of antimicrobial resistance genes alone or in combination. Meanwhile, the resultant IncHI2-positive isolates were tested harboring the three mobile genetic elements. After screening for antimicrobial susceptibility, 61 isolates were detected simultaneously harboring IncHI2 plasmid, IS26 and class 1 Integron, and 59 were confirmed as multidrug resistant, to Sulfisoxazole (100%), Ampicillin (90.16%), and Trimethoprim-Sulfamethoxole (90.16%). All these results warned us against the high risk of IncHI2-mediated multiple antimicrobial resistance dissemination in S. enterica.