報告題目：Closed-loop real-time all-optical interrogation of neural circuits in vivo
報  告 人：Michael Häusser教授
報告時間💅🏼🏓：3月26日  13:30-14:30
報告地點👌🏻：閔行校區生物藥學樓1號樓105會議室
聯  系 人：李衛東 liwd@sjtu.edu.cn

報告簡介：Neural activity patterns in the intact brain are rapidly evolving and highly variable from trial to trial. It is therefore crucial to be able to read out and interfere with these patterns in real time. Such closed-loop interventions would provide direct causal links between specific activity patterns and behaviour. I will describe a closed-loop all-optical strategy for dynamically controlling neuronal activity patterns in awake behaving mice. This approach involves rapidly tailoring and delivering two-photon optogenetic stimulation based on readout of activity using simultaneous two-photon imaging of the same neural population. I will show how this closed-loop feedback control can be used to clamp spike rates at pre-defined levels, boost weak sensory-evoked responses, and activate network ensembles based on detected activity. I will also demonstrate that this optical yoking together of neighboring neurons can be used to induce long-term changes in network dynamics. This approach thus allows the rate, timing and plasticity of activity patterns in neural circuits to be flexibly manipulated ‘on the fly’ during behavior.

報告人簡介：Michael Häusser is Professor of Neuroscience at University College London and a Principal Research Fellow of the Wellcome Trust. He received his PhD from Oxford University under the supervision of Julian Jack. He subsequently worked with Nobel Laureate Bert Sakmann at the Max-Planck-Institute for Medical Research in Heidelberg and with Philippe Ascher at the Ecole Normale Superieure in Paris. He established his own laboratory at UCL in 1997 and became Professor of Neuroscience in 2001. He is interested in understanding the cellular basis of neural computation in the mammalian brain using a combination of experiments and theory, with a special focus on the role of dendrites. His group has helped to pioneer several new optical approaches for probing the function of neural circuits in the intact brain.